A 256×256 CMOS Microelectrode Array for Extracellular Neural Stimulation of Acute Brain Slices

📋 论文概要

本文提出了一种256×256 CMOS微电极阵列，用于对急性脑切片进行细胞外神经刺激。该阵列解决了传统刺激方法空间分辨率低、刺激位点数量有限的问题，实现了高密度、高吞吐量的神经刺激。

💡 主要创新点

• 首次实现了256×256像素的高密度CMOS微电极阵列，用于神经刺激，相比传统被动MEA具有更高的空间分辨率和刺激位点数量。
• 阵列集成在CMOS芯片上，可实现每个电极的独立寻址和刺激控制，提高了刺激的灵活性和精度。

📄 原文摘要

investigating the function of the nervous system. Optical techniques, based on voltage- and calcium-sensitive dyes or photouncaging, along with multi-photon fluorescent microscopy have proven very successful in imaging activity in slices and in vivo. However, studies have been limited by the ability to stimulate different regions of tissue with enough spatial resolution and throughput. Traditional stimulation is accomplished with passive multielectrode arrays (MEAs) or bipolar electrodes. In both cases, a relatively small number of stimulation sites with coarse spatial resolution are possible. While there has been recent work on the development of CMOS chips for extracellular recordings of cultured neurons or slices on planar electrodes [1], the focus of this work is on stimulation and achieving stable electrical interfaces between acute slices

👥 作者与机构

Na Lei, Brendon O. Watson, Jason N. MacLean, Rafael Yuste, Kenneth L. Shepard

Columbia University, New York, NY Extracellular stimulation of neurons is an important tool in