A Microsystem for Time-Resolved Fluorescence Analysis using CMOS Single-Photon Avalanche Diodes and Micro-LEDs

📋 论文概要

该论文提出一种集成CMOS单光子雪崩二极管（SPAD）和微型LED的微系统，用于时间分辨荧光分析，解决了传统荧光测量依赖昂贵大型仪器且易受干扰的问题，实现了小型化、低成本和高精度的荧光寿命检测。

💡 主要创新点

• 首次将CMOS SPAD阵列与微型LED集成在同一微系统中，实现片上时间分辨荧光检测。
• 利用SPAD的单光子灵敏度和时间门控能力，结合微型LED的快速脉冲激发，实现荧光寿命的直接测量，克服强度法易受光源和浓度影响的缺点。

🏷 关键词

📄 原文摘要

Erdan Gu2, David R. Renshaw1, Edoardo Charbon3, Martin D. Dawson2, Robert K. Henderson1 1 University of Edinburgh, Edinburgh, United Kingdom University of Strathclyde, Glasgow, United Kingdom 3 Ecole Polytechnique Fédérale, Lausanne, Switzerland 2 Although microfluidics and microarray technologies are revolutionizing the throughput, sensitivity and cost in many areas of biodiagnostics [1], they are still reliant on bulky and expensive fluorescence analysis instrumentation. Conventional fluorescence intensity measurements are prone to misinterpretation due to illumination and fluorophore concentration non-uniformities. Thus, there is a growing interest in time-resolved fluorescence detection, whereby the characteristic fluorescence decay time-constant (or lifetime) in response to an impulse excitation source is measured. We describe the first complete micro-scale time-resolved fluorescence

👥 作者与机构

Bruce R. Rae1, Chris Griffin2, Keith R. Muir1, John M. Girkin2,