CMOS Electro-Chemical DNA-Detection Array with On-Chip ADC

📋 论文概要

该论文设计了一款基于CMOS工艺的576电极微阵列芯片，用于无标记电化学DNA杂交检测。通过将电极-电解质电容作为一阶delta-sigma调制器的积分器，实现了紧凑的24通道并行读取和片上模数转换，解决了传统方法需要外接仪器和标记的问题。

💡 主要创新点

• 利用电极-电解质电容作为delta-sigma积分器，显著简化了读取电路并实现片上A/D转换。
• 集成576个电极和24通道并行读出，实现高密度、高通量的DNA检测。
• 采用无标记电化学检测方法，避免荧光标记的复杂性和成本。

🏷 关键词

📄 原文摘要

ETH Zurich, Zurich, Switzerland, 2Georgia Institute of Technology, Atlanta, GA We report on a CMOS-based microsensor array, featuring 576 electrodes and 24 channels, for label-free electrochemical detection of DNA hybridization. Each channel comprises a potentiostatic circuit that keeps the electrode at a defined potential and measures the respective electrical current. The readout channels rely on a firstorder delta-sigma architecture, where the electrode-electrolyte capacitance is used as the integrator. This results in a very compact recording circuit inclusive of A/D conversion; the circuit consists of only the measurement electrode, a comparator, a feedback capacitance and a few switches. Biological experiments with short DNA samples and DNA extracted from human immunodeficiency virus (HIV) are presented. There is an urgent need for rapid on-the-spot diagnosis of infectious diseases like HIV. A very common diagnostic method includes the use

👥 作者与机构

Flavio Heer1, Manuel Keller1, George Yu2, Jiri Janata2, Mira Josowicz2, Andreas Hierlemann1