A Fully Integrated CMOS Fluorescence Biochip for Multiplex Polymerase Chain-Reaction (PCR) Processes

📋 论文概要

该论文提出了一种全集成CMOS荧光生物芯片，用于多重聚合酶链反应（PCR）过程，旨在实现生物分子检测系统的微型化和集成化。通过将荧光检测与PCR扩增集成在单一芯片上，解决了传统方法中需要外部光学设备、体积庞大且通量低的问题。

💡 主要创新点

• 首次实现了CMOS工艺下完全集成的荧光检测与PCR功能，无需外部光学滤波和透镜系统。
• 支持多重PCR检测，能够同时扩增和检测多个核酸靶标序列，提高检测通量和效率。
• 通过片上集成光学滤波器和光电探测器，实现了高灵敏度、低功耗的荧光信号检测，适用于即时诊断应用。

🏷 关键词

📄 原文摘要

Bob Kuimelis, Sara Bolouki, Pejman Naraghi-Arani, Kirsten Johnson, Mark McDermott, Nicholas Wood, Piyush Savalia, Nader Gamini InSilixa, Sunnyvale, CA Integration and miniaturization of bio-molecular detection systems into electronic biosensors and lab-on-chip platforms is of great importance. One widely recognized application area for such devices is nucleic acid (DNA and RNA) detection, specifically, nucleic acid amplification testing (NAAT), which relies on enzymatic processes such as polymerase chain reaction (PCR) to increase the copy number of target sequences and detecting them spectroscopically [1,2]. Here, we present a fully integrated CMOS DNA biosensor array (biochip) for clinical NAAT capable of performing multiplex (parallel) PCR in one ~40μL reaction chamber using on-chip thermo-cycling (±4°C sec-1 heat/cool rate), real-time amplicon-probe hybridization detection (up to target 1000 unique sequences),

👥 作者与机构

Arjang Hassibi, Rituraj Singh, Arun Manickam, Ruma Sinha,