A 16384-Electrode 1024-Channel Multimodal CMOS MEA for High-Throughput Intracellular Action Potential Measurements and Impedance Spectroscopy in Drug-Screening Applications

📋 论文概要

该论文提出了一种具有16384个电极和1024个通道的多模态CMOS微电极阵列（MEA），用于高通量细胞内动作电位测量。它解决了传统膜片钳技术通量低的问题，能够同时记录大量细胞的电生理活动。

💡 主要创新点

• 实现了16384个电极和1024个通道的高密度集成，显著提高了测量通量。
• 支持多模态测量，能够同时进行细胞内和细胞外记录。
• 采用CMOS工艺，实现了低噪声和高空间分辨率。

🏷 关键词

📄 原文摘要

Jan Putzeys1, Carl Van Den Bulcke1,3, Jan-Willem Weijers1, Andrea Firrincieli1, Veerle Reumers1, Dries Braeken1, Nick Van Helleputte1 imec, Heverlee, Belgium Chrysalite, Tervuren, Belgium 3 KU Leuven, Leuven, Belgium 1 2 Patch clamp is currently the gold standard for studying cell electrophysiology in preclinical drug discovery. Since it is a time-consuming technique, passive and active multielectrode arrays (MEAs) have been introduced for increased throughput in extracellular (ExC) in vitro measurements [1-3]. However, most of these tools cannot capture the essential features of intracellular (InC) action potentials (APs) used for studying drug toxicity. It has been shown that InC access can be achieved by highly localized electroporation [4], which allows lowimpedance electrical recording of the InC voltage. While this technique was explored in recent designs [4-5], a tool that enables high-throughput InC

👥 作者与机构

Carolina Mora Lopez1, Ho Sung Chun1, Laurent Berti2, Shiwei Wang1,