A 65nm CMOS Hydrogel-Based Dual Fluorescence Sensor for Bioavailable Phosphorus Detection

📋 论文概要

该论文提出了一种基于水凝胶的双荧光传感器，用于检测生物可利用磷，解决了环境监测中磷检测的灵敏度和动态范围问题。传感器实现了6.6fA的灵敏度、15.5dB的信噪比和1.97mV的输出噪声，并通过自动背景校准实现了大动态范围。

💡 主要创新点

• 首次将水凝胶与双荧光检测结合，用于生物可利用磷的实时传感。
• 采用高精度自动背景校准技术，通过粗调和细调控制环实现大动态范围。
• 利用单培养双荧光方法同时检测磷限制和细胞活力。

🏷 关键词

📄 原文摘要

*Equally Credited Authors (ECAs) Abstract A hydrogel-based single-culture dual-fluorescence sensor is presented for bioavailable phosphorus (P) detection. The sensor achieves 6.6fA sensitivity with a 15.5dB SNR and an output noise of 1.97mV at 182ms integration time. A large dynamic range is enabled through high-precision automatic background calibration with a coarse and fine control loop. The sensor was validated using hydrogels embedded with a bioreporter strain and successfully detected both P limitation and cell viability. Dynamically sensing bioavailable analytes in the environment is currently a very challenging task. For example, phosphorus (P), an essential nutrient for ecosystem health, is typically monitored on active farms at the frequencies of once every several years. Elevated P levels from agricultural runoff can lead to eutrophication in water bodies, while P deficiency in

👥 作者与机构

Ting-Yu Cheng*, Raj S. Mukkamala*, Reinaldo E. Alcalde, Mohamadamin Panahandeh, Akshit Agarwal, Julia A. Kornfield, Dianne K. Newman, Azita Emami

California Institute of Technology, Pasadena, CA